Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. FlexStation 3 Multi-Mode Microplate Reader. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. 5. 00. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. SpectraMax iD5. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). A Vision for the Future With TTP LabTech. CloneMatrix. Vertical Jump. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Page: 1/4 NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 4) onto the FilterMax F5 to be read with SpectraMax® Paradigm® Multi-Mode Detection Platform. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. Synergy HTX laboratory equipment pdf manual download. com 8. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018. Place plate in the GloMax® Instrument and read. Citations: Dual monochromators increase performance and assay flexibility. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. 8 Pages. Renilla/Firefly luciferase activities were measured twice. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Categories:. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). 4. Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. Table of Contents. Seoul, South Korea. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. The colorimetric signal development was stopped after 10 minutes. The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. 4 Pages. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). aureus , the amount of bacteriophage-MBs and the dilution ratio of the HRP. This can increase throughput and efficiency, thus saving time and space in the lab. Delete from my manuals. Search LabX. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. ” 3. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 4. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 4 Pages. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. 012 fmol 0. In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. Supernatant (25 μL), or standard, was added and mixed. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. 3 Pages. 00. The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. - September 14, 2010 - Molecular Devices, Inc. 425 or. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 3. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Instrumentation and Quality Control. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. Yes, the M5 does have a limited slip. Mettler Jr. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Multi-Mode Reader Comparison Table; Single Mode Readers. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. 0´ 104 1. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. 8 Pages. Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. Neurotransmitter Transporter Uptake Assay Kit. 4 Pages. Other technologies such as imaging. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. BMG LABTECH. d. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). 2 Pages. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. The results are expressed as cell viabilities (%) based on the control condition. The following document is intended to demonstrate setup of this instrument. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Align the two slots on the bottle holder with the two pegs on the rail. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). $3,000 USD. In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular. Ease of use. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Neurotransmitter Transporter Uptake Assay Kit. 3. The USB adaptor comes with 1 X CD that contains its USB driver. For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. Besides the number of substrates used by microbiota,. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Hit enter to search or ESC to close. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). CloneMatrix. Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. Please click on the links below to view the Microplate Reader Comparison Tables. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. 2. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. Different parameters (for instance, the incubation time of bacteriophage-MBs and S. Catalog. 3. The values of luminescence are directly proportional to the number of viable cells. 00 USD. 00. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Learn More. 00. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. FilterMax F5 Multi-mode microplate reader. 3 Pages. Click to Contact Seller. Figure 2-23: Bottle holder To install the bottle holder: 1. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. , call 1-800-635-5577. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Transfluor Technology. The mixture was then boiled for 10 min. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. 3 Pages. 4 Pages. Methods for MycoAlert and MycoAlert PLUS Assays are basically the same, except for the concentration range of assay control used, and are detailed below. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. BMG Labtech - FLUOSTAR OMEGA. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. Match case Limit results 1 per page. The OD 595 of the culture in the plate (light path length: ∼6. Upgradeable modules are also available including western blot, cell imaging, and fast. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. 1. x. Time-resolved Fluorescence. 8 Pages. , Sunnyvale, CA, USA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. book Page 1 Tuesday, September 7, 2010 6:14 PM. Detection modalities are shown in Table 1-1. prt Read the plate and then save the experiment. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. CloneMatrix. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. F5 Multi-Mode Microplate Reader. The papers, which were published between 2010 and. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. 3 Pages. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The experiment was performed in 4 technical replicates (wells in the cell culture plate). Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. SpectraMax Glo Steady-Luc Reporter Assay Kit. Multi-mode Microplate Readers. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 50/50. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Renilla/Firefly luciferase activities were measured twice. 7°C and the bottom to 36. 3 Pages. #6590-0118) Filter 665/10 (Molecular Devices cat. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Click Run. 3 Pages. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. 425 or. Figure 1. 3 Pages. Fluorescence was measured with excitation and emission wavelengths of 530 and 590 nm, respectively, on a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. 3 Pages. MDA is one of the final products of LPO. 5. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. ** Some compatible filter sets are available for. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. 2. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. ; Page 2 For research use only. F5 Multi-Mode Microplate Reader. MetaMorph NX. The door opens, revealing the transport lock that fastens the microplate drawer to the internal frame of the instrument. MetaFluor Software Brochure. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 3. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. 97059-524EA 84727. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. 2 Pages. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. The Labsystems Nepheloskan Ascent Microplate Reader is designed to run with 96-well microplates, for use on applications like immunoprecipitation reactions as well as solubility tests of pharmaceutical components. Important things to consider when purchasing a multimode microplate reader. ScanLater Western Blot Detection System. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. com A. 380 or. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). 020 fmol 0. 40266 Email: drugdiscoverytech@lifetech. The unique patented design ensures precise performance and sensitivity across all detection modes. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. c. FilterMax F5 Multi-mode microplate reader. 3 Pages. The data were analyzed using Multi-Mode Analysis software and normalized to fluorescence in the vehicle-treated cells (% of control). Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. SpectraMax Gemini XPS/EM Microplate Readers. FilterMax F3 & F5 Microplate Readers. Optical System: High energy xenon flash lamp. MS-3 Laser Bar Code Reader. 00 USD. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). SpectraMax Glo Steady-Luc Reporter Assay Kit. Luminescence intensity was measured with a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Sunnyvale, Calif. Add to cart. performs within its specifications. Measurements of Caspase-3/7 Activity. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. 4 Pages. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. Close Search. Transfluor Technology. Serum concentrations of CAF were determined according to manufacturer guidelines in duplicate via enzyme-linked immunosorbent assay (Glory Science Co. This instrument has passed specific verification criteria. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Assay buffer and 100% DMSO were used as vehicle controls. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). please click here. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. IMAP assays are based on binding of. Download Table of Contents. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. In . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 2 Pages. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. 3 Pages. 5% (v/v) to avoid toxicity. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 3 software (Molecular Devices). ] Example of document to evaluate if the reader . ( C ) H2A. 005 OD. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . View specifications, prices, citations, reviews, and more. Trinean Dropsense 96 Polychromatic. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). F5 Multi-Mode Microplate Reader. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. In the case of cocultures, donor and recipient. 2 Pages. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 00 USD. 3. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Synergy HT laboratory equipment pdf manual download. The drawing shows a standard microplate of 15 mm height covered with a lid. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. 4 Pages. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). Watson. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. 2. Owner, Zeckhausen. 561. ScanLater Western Blot Detection System. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. The OD 600 was plotted against time using Graphpad Prism 8. This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. 2 Temperature and cooling rate control. MICROPLATE READERS Multi-Mode Readers. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. 6. 2. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. CloneMatrix. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. shopping cart 0 items $0. The quantity of colorimetric product was continuously monitored for 120 min. Devices FilterMax Multi-Mode Microplate Readers.